FIG. 4.

S-HDAg produced from HDV RNA-replicating cells is a SUMO1 target protein. In Huh-7 cells, HDV genomic RNA was transfected together with the S-HDAg-encoding mRNA to establish HDV RNA replication. Four days after RNA transfection, the cells were then transfected again, with or without the SUMO1-GG and Ubc9 expression plasmids. Two days later, the whole-cell extracts were prepared and then subjected to immunoblotting with a mouse monoclonal antibody against HDAg (lanes 1 and 2) or with a rabbit polyclonal antibody specific for L-HDAg (LP3) (49) (lanes 3 and 4).