FIG. 7.

The SUMO1-S-HDAg fusion protein displays a subcellular localization pattern similar to that of wild-type S-HDAg. The mRNA encoding wild-type S-HDAg (wt) or mutant SUMO1-S-HDAg (SUMO-HDAg) was transfected into Huh-7 cells. At day 1 posttransfection, cells were fixed and prepared for immunofluorescence microscopic examination (as described in Materials and Methods). (A) A mouse anti-HDAg monoclonal antibody or a rabbit anti-SUMO1 polyclonal antibody was used as the first antibody. (B) A rabbit anti-HDAg polyclonal antibody or a mouse anti-nucleolin monoclonal antibody was used as the first antibody.