FIG. 4.
Antitumor efficacy of 105 PFU and 10 PFU of antiangiogenic vaccinia virus in a nude mouse model. (A) Doses of 5 × 104 or 5 PFU of vaccinia virus were injected intratumorally into subcutaneous 786-O tumors in nude mice (three mice per group; two tumors per mouse), and tumor size was plotted relative to the size before virus injection on day 1. (B) In another experiment nude mice (three mice per group; two tumors per mouse) were intravenously injected with 105 or 10 PFU of vaccinia virus, and tumor volumes were followed. (C) VEGFR-1-Ig concentration was measured in the blood of treated mice. (D) At the end of the experiment tumors were collected, sections were made and stained with polyclonal von Willebrand factor antibody for blood vessels (green), and pictures of representative areas showing the highest blood vessel densities were taken at 20× magnification. Bars indicate standard errors.