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. 2009 Nov 4;84(2):753–764. doi: 10.1128/JVI.01596-09

FIG. 2.

FIG. 2.

Inhibition of viral production by ex vivo-sorted, unstimulated, SIV-specific CD8+ T cells. (a) Viral RNA copies per milliliter of supernatant from cultures containing SIV-infected CD4+ T cells only (⧫), SIV-infected CD4+ T cells and Gag181-189CM9-specific CD8+ T cells (▾), and SIV-infected CD4+ T cells and Env573-581KL9-specific CD8+ T cells (▴). Supernatant was removed at 0, 24, and 48 h postinfection in duplicate or triplicate. (b) Antiviral hierarchy of SIV-specific CD8+ T cells specific for 12 epitopes. All assays were performed at an effector-to-target cell ratio of 1:1. Each data point represents the average percent maximum suppression capacity of one CD8+ T-cell specificity from one animal, with the black bar indicating the average percent maximum suppression of each specificity. Elite controllers (blue) have viral loads of <10e3 viral copies/ml, controllers (red) have viral loads between 10e3 and 2 × 10e4 viral copies/ml, and progressors (black) have viral loads of >2 × 10e4 viral copies/ml at the date of assay. (c to f) Percent maximum suppression capacity of SIV-specific CD8+ T cells at various effector-to-target cells ratios. Each data set is the average maximum suppression capacity of one CD8+ T-cell specificity from one animal. (c) SIV-specific CD8+ T cells directed to Gag. ⧫, Gag71-79GY9; +, Gag181-189CM9; ▴, Gag71-79GY9; ▾, Gag181-189CM9. (d) SIV-specific CD8+ T cells directed to Nef. ▴, Nef159-167YY9; •, Nef137−146RL10; ⧫, Nef137-146Rl10; ▾, Nef137-146RL10. (e) SIV-specific CD8+ T cells directed to Env. •, Env573-581KL9; ▴, Env524-532KF9; ◊, Env573-581KL9; ▾, Env868-876RL9; ⧫, Env573-581KL9; +, Env524-532KF9; ×, Env620-628TL9. (f) SIV-specific CD8+ T cells directed toward Vif, Tat, or Rev. ⧫, Vif123-131RL9; +, Tat28-35SL8; •, Vif172-179RL8; ▾, Tat28-35SL8; ▴, Rev12-20KL9.