FIG. 6.
Characterization of the infectivity and nuclear import abilities of the ΔcPPT-CTS mutant upon HIV-1 X4 Env pseudotyping. CA-normalized quantities of wild-type and ΔcPPT-CTS virions were pseudotyped with the HIV-1NL4-3 X4 envelope by transfection with appropriate coding DNAs, and purified virions were used to infect PHA-IL-2-stimulated PBLs. (A) The quantity of infected cells was assessed at 3 days postinfection by flow cytometry and is expressed as a percentage of the wild-type level. (B and C) PCR analysis was carried out as described for Fig. 3, and the relative quantities of FL products of reverse transcription (black bars) and the 1-LTR-circle/FL-DNA (white bars) and 2-LTR-circle/FL-DNA (gray bars) ratios were determined. Graphs present values obtained in 3 to 5 independent experiments. The asterisk indicates statistically significant differences from the WT level (Student test; P < 0.05).