FIG. 2.
Evaluation of the HIV-1 cofactor activities of LEDGF/p75 deletion mutants. (a) Schematic representation of deleted regions. (b) Different LEDGF/p75 deletion mutants were stably expressed in LEDGF/p75 knockdown TL3 cells, and their protein levels were evaluated by immunoblotting using anti-LEDGF or anti-FLAG MAbs. Alpha-tubulin detection was used as a loading control. P and P/A represents PWWP and PWWP/AT hook, respectively. (c) Single-round HIV-1 infections of TL3-derived cells. Cells immunoblotted in b were challenged with HIVluc, and luciferase activity was determined 5 days later.