FIG. 5.
Chromatin-binding activity of LEDGF/p75 mutants. (a) Chromatin-binding assay. TL3 cells expressing different LEDGF/p75 mutants were fractionated into non-chromatin-bound fractions (S1 and P2) and chromatin-bound fractions (P1 and S2), and the presence of LEDGF/p75 in these fractions was evaluated by immunoblotting with an anti-FLAG MAb. An unfractionated total cellular fraction (T) was included as a control. (b) Salt extraction analysis of LEDGF/p75 mutants stably expressed in TL3 cells. The salt concentration that extracted the LEDGF/p75 WT from chromatin is marked with a rectangle.