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. 2009 Nov 4;84(2):740–752. doi: 10.1128/JVI.01043-09

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FIG. 6. — Interaction of LEDGF/p75 mutants with HIV-1 integrase. (a) Integrase protection assay. LEDGF/p75-deficient HEK293T cells stably expressing Myc-tagged HIV-1 integrase were either transiently transfected or not transfected with plasmids expressing the LEDGF/p75 WT (I) or mutants (II). Expression levels of LEDGF/p75 proteins and HIV-1 integrase were determined by immunoblotting with an anti-FLAG and anti-Myc MAbs, respectively. Detection of endogenous c-Myc was used as a loading control for these experiments. (b) Coimmunoprecipitation of HIV-1 integrase with the LEDGF/p75 ΔCR3 mutant. LEDGF/p75-deficient HEK293T cells were cotransfected with the FLAG-tagged LEDGF/p75 WT or different mutants and Myc-tagged HIV-1 and subjected to immunoprecipitation with an anti-FLAG MAb. Immunoprecipitated proteins were detected by immunoblotting with anti-Myc or anti-FLAG MAbs. Mouse antibody light chains were used as a loading control for the immunoprecipitation.