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Integrase-to-chromatin-tethering assay. LEDGF/p75-deficient HEK293T cells stably expressing EGFP-tagged HIV-1 integrase were either transiently transfected or not transfected with plasmids expressing the LEDGF/p75 WT or mutants and analyzed by fluorescence microscopy. Chromatin was stained with DAPI.
