FIG. 3.
Characterization of chimeric GPs. (A and C) The titers of the vectors pseudotyped with parental GPs or indicated chimeras were determined on CEM cells as previously described (27). Values shown are the means plus SD (error bars) for three to six independent experiments. An asterisk indicates no detectable titer, even for 10× concentrated vector stocks. Chimeric GPs with altered characteristics that no longer reflect the parental GP backbone are indicated with a black arrowhead, and partial effects are indicated with a white arrowhead. (B and D) Relative titers of the indicated vectors on CHO-K1 cells transiently transfected with hTfR1 versus mock-transfected CHO-K1 cells. Values are the means plus SD for more than five independent experiments. Chimeric GPs with altered characteristics that no longer reflect the parental GP backbone are indicated with a black arrowhead. VSV, vesicular stomatitis virus.