FIG. 1.

Mapping of the hhi1 transcript of HzNV-1 virus. (A) Mapping of hhi1 transcript. The first line shows the corresponding sequence numbers, 609 bp and 217820 bp on the complementary strand, of the nucleotides of HindIII-I fragment (5). The locations of a TATA box-like sequence, initiation site of transcription (+1), and translational start codon (ATG) on the HindIII-I fragment are indicated. (B) Temporal expression profile of the hhi1 gene. Sf-21 cells were infected with HzNV-1 virus at an MOI of 10. Total RNA was extracted and resolved on agarose gel and analyzed by Northern blot analysis using an hhi1-specific probe generated from a DIG labeling reaction. Lane 1, total RNA extracted from mock-infected cells; lanes 2 to 9, total RNA extracted from infected Sf-21 cells at 0.5 to 12 hpi; lane 10, total RNA extracted from latently infected SFP4 cells.