FIG. 5.

Induction of viral reactivation by the hhi1 gene in latent cells. (A) The map of plasmids pKShH1 and pKShE. In these two plasmids, hhi1 and egfp coding regions were driven by the heat shock promoter. (B) Change of cell morphologies over time after plasmid transfection. Latently infected SFP4 cells were transfected with plasmids pKShH1 (a to c) and pKShE (e to g), and cell images were taken at different time points posttransfection. (C) Viral titers estimated from productively and latently infected cells. Latently infected cells were transfected with plasmids pKShH1 and pKShE separately, and the titers of the released viruses were determined at different time points. Sf-21 cells were infected with wild-type virus (HzNV-1) as a control.