FIG. 8.

Effect of brefeldin A on membranous web-like structure formation in UHCV-11 cells. (A) UHCV-11 cells were induced by tetracycline withdrawal and incubated for 8 h in the presence of different concentrations of BFA or ethanol (0.2%). Control cells were incubated for 8 h without induction (tetracycline). HCV protein expression was assessed by immunoblot analysis of E1, NS5A, and tubulin. (B) Cells induced for 8 h in the presence or absence of BFA (1 μg/ml) were fixed and processed for immunofluorescence detection of NS5A (red) and the Golgi marker GM130 (green) Nuclei were stained with DAPI (blue). Bar, 20 μm. (C) Cells induced for 8 h in the presence or absence of BFA (10 μg/ml) were fixed and processed for electron microscopy detection of membranous web-like membrane alterations. Bars, 0.5 μm.