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. 2009 Nov 4;84(2):1097–1109. doi: 10.1128/JVI.01662-09

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FIG. 7. — 3a vesicles in live cells contain endosomal markers and are acidic. (A) Vero cells were cotransfected with 3a-GFP, TGN38-CFP, and Cherry-Lamp1, and live cells were analyzed by confocal microscopy at 24 hpt. Arrows indicate examples of vesicles that contain 3a, TGN38, and Lamp1. (B) Vero cells were transfected with 3a-GFP and analyzed by confocal microscopy 24 hpi after counterstaining with LysoTracker Red DND 99 according to the manufacturer's instructions. Two examples of transfected cells are shown. Areas of colocalization appear orange in merge image.