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. 2009 Nov 11;84(2):822–832. doi: 10.1128/JVI.01339-09

FIG. 6.

FIG. 6.

Histone H2B is a crucial element for suppression of DNA virus replication. (A to E) HEK293 (A and C), SCC-4 (B), NIH 3T3 (D), or HeLa cells (E) were pretreated with control siRNA or H2B siRNA. (A) The cells were infected with MVAdE3L (1 × 105 or 1 × 107 PFU). Twenty-four hours after infection, cell lysates were subjected to immunoblotting for total STAT1, phosphorylated STAT1 (p-STAT1), or ERK. (B) The cells were transfected with the HPV11 or -16 genome, and the episomal DNA fractions were recovered 24, 36, 60, or 72 h after transfection. Viral multiplication was determined by PCR. (C and D) The cells were infected with AdV type 5 or MCMV strain MW97.01. Twenty-four hours after infection, viral multiplication was determined by PCR amplification of the viral genomic DNA and by a plaque assay (AdV) or by measuring the TCID50 (MCMV). (E) The cells were infected with EMCV. Twenty-four hours after infection, viral multiplication was determined by a plaque assay. *, P < 0.05; **, P < 0.01.