FIG. 2.

Efficiency of STAT1-GFP nuclear accumulation in cells expressing wild-type and mutant Z-VP24 proteins. (A) Vero E6 cells were transfected with plasmids encoding STAT1-GFP plus either pCAGGS (empty vector), pCAGGS-HAVP24 (encoding wild-type VP24), pCAGGS-HAmut1, pCAGGS-HAmut2, or pCAGGS-HAmut3. At 16 h posttransfection, cells were mock treated or treated with 1,000 U/ml IFN-β for at least 30 min. Cells were then fixed with paraformaldehyde and stained for expression of VP24. Nuclei were stained with dapi (4′,6-diamidino-2-phenylindole). (B) The graph represents the percentages of cells showing nuclear STAT1-GFP (white) or cytoplasmic STAT1-GFP (black) in the presence of different VP24 proteins.