FIG. 5.

Suppression of HCV replication restores sensitivity to SPDCA. G54 cells were treated with different doses of the HCV inhibitor ribavirin. (A) Suppression of HCV replication and SPIK expression was examined by Northern blotting, using HCV- and SPIK-specific probes as previously described. (B) Resistance of G54 cells to SPDCA was quantified by flow cytometry after treatment with BFA-CHX-Z-VAD. Apoptotic cells are represented by peaks with higher fluorescence. The percentage of apoptotic cells is indicated. (C) The effect of ribavirin treatment on cell viability was examined by a WST test kit after treatment of G54 cells with increasing concentrations of ribavirin.