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. 2009 Oct 28;84(1):96–108. doi: 10.1128/JVI.01475-09

FIG. 2.

FIG. 2.

Comparison of STAT5 phosphorylation and CD127 expression between CD4+ T-cell subsets in healthy donors. PBMC from healthy donors (n = 13) were stimulated with 5 pM IL-7 (A) or 500 pM IL-7 (B) for 15 min and evaluated for pSTAT5 induction by flow cytometry within the four CD4+ T-cell subsets (NTreg, MTreg, Nv, and Mem). “ΔpSTAT5 MFI” represents the difference in pSTAT5 MFI between IL-7-stimulated and unstimulated samples. (C) PBMC from healthy donors (n = 19) were evaluated for CD127 expression within the four CD4+ T-cell subsets. (D) PBMC from healthy donors (n = 11) were evaluated for CD132 expression within the four CD4+ T-cell subsets. Statistical analyses were performed with the nonparametric Mann-Whitney U test. A Bonferroni correction was applied because six comparisons were made between four populations. Therefore, the significance threshold was lowered to a P value of <0.0084 (0.05/6). All P values of <0.0084 are reported. Horizontal bars indicate median values.