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. 2009 Oct 28;84(1):96–108. doi: 10.1128/JVI.01475-09

FIG. 4.

FIG. 4.

Decreased binding of IL-7 to memory CD4+ T cells from viremic patients. (A) Binding of biotinylated IL-7 to nonpermeabilized PBMC was evaluated by flow cytometry within the naive (CD3+ CD4+ CD45RA+; top panel) and the memory (CD3+ CD4+ CD45RA−; bottom panel) CD4+ T-cell compartments. Bound IL-7 was revealed by incubation with avidin-FITC (Fluorokine assay; R&D Systems). Binding levels were compared between PBMC from healthy donors (n = 7; clear circles) and those from viremic patients (n = 4; solid squares). Error bars represent standard deviations. (B) IL-7 binding values obtained at the highest IL-7 dose (40 ng) were compared between healthy donors and viremic patients, within naive CD4+ T cells (top panel) and memory CD4+ T cells (bottom panel). The negative controls consisting of samples incubated with a blocking IL-7 antibody (+anti-IL-7) and biotinylated IL-7 are represented on the right of the graphs.