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Immunoelectron microscopy of v62i virus particles. COS cells were infected with BA71V and with the recombinant v62i in the absence of IPTG. At 24 hpi, the cells were fixed and processed by freeze-substitution. Ultrathin Lowicryl sections were incubated with antibodies against the polyprotein pp220-derived protein p150 (pp220) and polyprotein pp62-derived protein p35 (pp62), followed by incubation with protein A-gold (10-nm diameter). Gold grains are indicated by arrows. Bars, 100 nm.
