TABLE 2.
Quantification of polyproteins pp62 and pp220 within the different types of virus particles by immunoelectron microscopya
| Antibody and virus | Mean no. ± SEM/% of gold grains in particles |
|||
|---|---|---|---|---|
| Empty | Immature | Defective | Mature | |
| Anti-pp62 | ||||
| BA71V | ND | 3.37 ± 0.28/59.5 | ND | 5.67 ± 0.36/100 |
| v62i (without IPTG) | 0.04 ± 0.02/0.7 | 0.22 ± 0.03/3.8 | 0.96 ± 0.16/17.0 | ND |
| Anti-pp220 | ||||
| BA71V | ND | 3.82 ± 0.34/58,5 | ND | 6.53 ± 0.40/100 |
| v62i (without IPTG) | 0.43 ± 0.08/6.6 | 2.62 ± 0.19/40.1 | 3.98 ± 0.36/60.9 | ND |
a
Immunogold labeling was performed as described in the legend to Fig. 7. Quantification was performed for closed hexagonal profiles at the assembly sites. For each condition and antibody, gold grains within at least 100 particles of each type were counted. Percentages are with respect to the mean number of gold grains detected in the mature BA71V particles. ND, not determined.