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Inhibitor effect on genotype 1a/1b NS5A hybrids. DNAs encoding various HCV replicons were expressed in a vaccinia virus transient expression system treated with either DMSO (−) or 0.1 μM BMS-824 (+). Cell lysates were separated by SDS-PAGE on 8% gels, and NS5A proteins were identified by Western immunoblotting using an anti-NS5A antibody. Cmpd, compound.
