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. 2009 Oct 21;84(1):585–598. doi: 10.1128/JVI.01748-09

FIG. 4.

FIG. 4.

Ectopic expression of ZBP1 increases UV-HCMV-induced IFN-β transcription independently of IPS-1. (A) Immunoblot showing overexpression of ZBP1 in two stable THF lines. (B) Induction of IFN-β mRNA as measured by qPCR in UV-HCMV-exposed relative to unexposed cells; the values shown are normalized to the IFN-β induction observed in THF parental cells (set to 1). (C) Immunoblot showing siRNA-mediated knockdown of stably expressed ZBP1 from THF-ZBP1 cells using two different ZBP1-targeted siRNA sequences. (D) Induction of IFN-β mRNA as measured by qPCR in UV-HCMV-exposed relative to unexposed THF-ZBP1 cells following treatment with the indicated siRNAs; the values shown are normalized to the IFN-β induction observed in THF-ZBP1 cells treated with NS siRNA (set to 1). (E) IFN-β secretion as measured by luciferase expression from THF-ISRE cells following exposure to media collected from THF or THF-ZBP1 cells that were either untreated or treated with UV-HCMV. The values (plus standard errors of the mean) are normalized to untreated THFs that were not exposed to UV-HCMV (set to 1). *, P < 0.05.