FIG. 3.

Coreceptor usage of viruses recovered from macaques DG08, DR16, and DN57 at time of overt AIDS. Coreceptor utilization, determinants, and preference were assessed by infection of U87.CD4.CCR5 (black bars) and U87.CD4.CXCR4 (striped bars) cells with SHIV viruses recovered from macaques DG08, DR16, and DN57 near the time of death (A); luciferase reporter viruses expressing the envelopes with WT or Δ22-25- or HR-bearing V3 loop sequence of macaque DG08 and the S11R V3 loop sequence of DR16 (B); and V3 chimeric pseudotyped viruses (C). (D) Blocking of entry of the indicated pseudotyped viruses into TZM-bl cells with 1 μM CCR5-specific (TAK779) and CXCR4-specific (AMD3100) inhibitors. Data in panels B, C, and D are the means and standard deviations from triplicate wells and are representative of at least two independent experiments. RLU, relative light units.