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Phylogenetic tree showing the relationship between Env variant sequences (V1 to V5) in macaque DG08. A neighbor-joining tree rooted on the sequences of the inoculating virus SHIVSF162P3N was generated. The scale bar indicates the genetic distance along the branches in nucleotides, and the asterisk along a branch represents a bootstrap value of >70%. ▪, representative clones of the inoculating virus; ▴, WT variants; •, HR-bearing variants; and ○, Δ22-25-bearing variants.
