TABLE 1.
HA sequences and reassortant virus titers of novel H1N1 variants
| WT virus (passage history) | 6:2 Reassortant variant | Amino acid at indicated positiona (H3 position) |
% of clones with change(s)c | Titerb of reassortant in eggs (log10 FFU/ml ± SE) | |||
|---|---|---|---|---|---|---|---|
| 191 (194) | 197 (200) | 222 (225) | 223 (226) | ||||
| A/CA/4/09 (MDCK, twice) | WT | L | T | D | Q | NA | NAc |
| V1 | 100 | Not rescued | |||||
| A/CA/4/09 (egg, twice) | V2 | I | 42 | 7.4 ± 0.09 | |||
| V3 | R | 50 | 7.8 ± 0.15 | ||||
| V7 | I | R | 8 | 7.8 ± 0.40 | |||
| A/CA/7/09 (egg, twice) | WT | L | A | D/G | Q/R | NA | NA |
| V4 | D | Q | 45 | 7.8 ± 0.27 | |||
| V5 | G | Q | 34 | 7.4 ± 0.14 | |||
| V6 | D | R | 21 | 7.7 ± 0.14 | |||
a
Amino acid changes from the wild type A/CA/4/09 or A/CA/7/09 are shown.
b
Virus titers are mean titers from at least three virus stocks.
c
NA, not applicable.