FIG. 7.

The IFN system is functional in primary melanoma cultures. Melanoma cultures 351 ADV (A) and 289 ADV (B) were either mock infected (filled black) or NDV-HUJ infected at an MOI of 10 (red line). Cultures were pretreated with human IFN-β (1,000 U/ml) for 16 h before NDV (purple line) or mock (blue line) infection. Cells were harvested 72 h postinfection and stained with anti-NDV antibodies. Surface NDV protein was quantified using the FGM value, as described in Materials and Methods. (C) Cell death was analyzed by flow cytometry of PI-stained 289 ADV, 351 ADV, and 298 ADV melanoma cultures as described in Materials and Methods.