TABLE 3.
Comparison of IC50s for CD4 reagents and commonly studied broadly neutralizing anti-HIV-1 Abs
| Clade and Env | IC50 (nM)a |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4HC-(GS7)-IgGE51 | CD4HC-(GS9)-IgGE51 | CD4HC,LC-(GS7)-IgGE51 | CD4HC-(GS7)-IgG17b | CD4HC-(GS7)-IgGm9 | CD4HC-(GS7)-IgG412d | CD4HC-(GS7)- IgGanti-gD | CD4-IgG2 | CD4-Fc | CD4 | 4E10 | b12 | 2G12 | 2F5 | PG9 | PG16 | |
| Clade B | ||||||||||||||||
| SF162.LS | 0.37 | <0.26 | ND | <0.26 | ND | ND | ND | ND | ND | 2.3 | 0.07 | 0.06 | 4.0 | 0.68 | >330 | >330 |
| PVO.4 | 12 | 7.1 | 9.8 | 7.0 | 27 | 14 | 5.2 | 27 | 300 | 310 | 45 | >330 | 8.0 | >330 | 27 | 36 |
| CAAN5342.A2 | 14 | 6.8 | ND | 7.5 | 110 | 90 | 25 | 41 | 870 | 750 | 19 | >330 | >330 | 24 | 38 | 59 |
| WITO4160.33 | 3.2 | 1.6 | 5.5 | 1.4 | 17 | 8.2 | 4.6 | 8.0 | 120 | 250 | 2.1 | 20 | 7.3 | 4.1 | ||
| AC10.2.29 | 7.3 | 2.8 | >420 | 3.5 | 51 | ND | 14 | 11 | 230 | 400 | 2.1 | 12 | >330 | 8.8 | ||
| SC422661.8 | 7.0 | 5.7 | 10 | 7.4 | 28 | 14 | 6.7 | 14 | 240 | 220 | 6.2 | 1.3 | 14 | 4.8 | 5.3 | 7.5 |
| 6535.30 | 1.7 | 1.1 | 9.8 | 0.8 | 6.8 | ND | 1.5 | 13 | 29 | 37 | 1.4 | 9.1 | 13 | 13 | 1.5 | 250 |
| THRO4156.18 | 2.8 | 1.8 | ND | ND | 4.7 | 5.1 | 2.6 | 2.3 | 12 | 14 | 2.1 | 3.2 | >330 | >330 | 83 | 8.9 |
| REJO4541.67 | 2.3 | 1.4 | 30 | ND | 8.4 | ND | 3.1 | 2.9 | 17 | 23 | 4.9 | 4.5 | >330 | 4.1 | ||
| TRJO4551.58 | 20 | 11 | ND | ND | 66 | ND | 35 | 19 | 500 | 940 | 31 | >330 | >330 | >330 | 2.9 | 7.7 |
| QH0692.42 | 3.1 | 1.8 | 16 | ND | 10 | 9.7 | 1.0 | 2.3 | 14 | 23 | 9.7 | 1.9 | 19 | 6.8 | >330 | >330 |
| TRO.11 | 86 | 52 | 4.7 | ND | 31 | ND | 9.3 | 45 | 770 | 540 | 2.1 | >330 | 2.7 | >330 | 36 | 1.5 |
| RHPA4259.7 | 3.2 | 1.5 | 370 | ND | 34 | 20 | 5.2 | 5.2 | 82 | 84 | 48 | 0.6 | >330 | 82 | ||
| Clade C | ||||||||||||||||
| MW965.26 | <0.26 | <0.26 | ND | <0.26 | ND | ND | ND | ND | ND | |||||||
| ZM197M.PB7 | 22 | 12 | >420 | 7.2 | 61 | 63 | 35 | 91 | >1,000 | 180 | 3.5 | 130 | >330 | 84 | ||
| ZM249.PL1 | 13 | 4.6 | ND | 4.1 | 83 | 42 | 19 | 30 | 1000 | 450 | 15 | 21 | >330 | >330 | ||
| ZM53M.PB12 | 2.8 | 1.1 | ND | 1.5 | 28 | 31 | 4.6 | 12 | 350 | 390 | 49 | 170 | >330 | >330 | ||
| ZM214M.PL15 | 7.0 | 6.0 | >420 | 5.2 | 22 | 16 | 13 | 16 | 470 | 370 | 28 | 19 | >330 | >330 | ||
| Du156.12 | 51 | 35 | >420 | 35 | 120 | 53 | 87 | 40 | >1,000 | 630 | 1.4 | 5.2 | >330 | >330 | ||
| Du422.1 | 1.6 | 0.8 | >420 | 1.3 | 150 | 85 | 68 | 40 | 630 | 420 | 4.9 | 1.3 | >330 | >330 | ||
| Du172.17 | 5.2 | 2.9 | 160 | ND | 21 | 28 | 8.8 | 8.6 | 56 | 79 | 2.1 | 6.5 | >330 | >330 | ||
| CAP45.2.00.G3 | 3.6 | 2.0 | ND | ND | 46 | 11 | 20 | 6.3 | >1,000 | 1200 | 18 | 4.5 | >330 | >330 | ||
| CAP210.2.00.E8 | 6.3 | 2.9 | ND | ND | 18 | 20 | 7.7 | 3.4 | 50 | 160 | 8.3 | 130 | >330 | >330 | ||
| ZM233M.PB6 | 6.6 | 2.3 | ND | ND | 42 | 40 | 11 | 33 | 460 | 140 | 8.3 | >330 | >330 | >330 | ||
| ZM109F.PB4 | 0.52 | <0.26 | ND | ND | 2.1 | 2.0 | 0.52 | 0.29 | 3.1 | 9 | 4.2 | >330 | >330 | >330 | ||
| ZM135M.PL10a | 13 | 9.3 | ND | ND | 71 | 43 | 22 | 8.0 | 420 | 280 | 4.2 | >330 | >330 | >330 | ||
| Clade A | ||||||||||||||||
| DJ263.8 | <0.26 | <0.26 | ND | <0.26 | ND | ND | ND | ND | ND | |||||||
| Q23.17 | 3.6 | 2.1 | >420 | 1.8 | 69 | 53 | 15 | 18 | 530 | 120 | >330 | >330 | 50 | |||
| Q842.d12 | 45 | 43 | >420 | 33 | 200 | 86 | 110 | 90 | >1,000 | 97 | >330 | >330 | 59 | |||
| Q259.d2.17 | 2.7 | 1.8 | >420 | 1.8 | 43 | 38 | 12 | 13 | 93 | 99 | >330 | >330 | 72 | |||
| 3718.v3.c11 | 19 | 9.4 | >420 | 19 | ND | 94 | 55 | 41 | >1,000 | 80 | >330 | >330 | 23 | |||
| 0330.v4.c3 | 2.4 | 1.3 | 9.3 | 1.0 | ND | 12 | ND | 13 | 130 | 40 | >330 | 4.7 | 65 | |||
| 3415.00 | 16 | 8.1 | 12 | 10 | ND | 18 | ND | 90 | 640 | 160 | 160 | 14 | 250 | |||
| Geometric mean | 5.0 | 2.9 | 75 | 2.9 | 32 | 25 | 11 | 14 | 210 | 150 | 10 | 33 | 110 | 78 | 27 | 33 |
| Arithmetic mean | 12 | 7.2 | 220 | 7.1 | 51 | 36 | 22 | 25 | 440 | 320 | 30 | 150 | 240 | 190 | 96 | 110 |
IC50s for the 4E10, b12, 2F5 Abs and CD4 were taken from previously reported results (21, 22) or were provided by the CAVD neutralization core. IC50s for 2G12 were taken from references 21 and 22 and converted to molar concentrations, assuming the monomeric form. PG9 and PG16 were tested on a larger panel of viruses (35), but only those that overlapped with our panel are reported in this table. Over a 162-pseudovirus panel, PG9 and PG16 had geometric mean IC50s of 5.7 and 5.5 nM, respectively. In calculating average IC50s, measurements outside of the range of the assay (indicated by > or < symbol) were assigned to that limiting value. ND, not determined.