FIG. 2.

Ligand-specific inhibition of ANDV infection. Potentially competitive ligands (5 to 20 μg/ml) were adsorbed to VeroE6 (A) and Syrian hamster BHK-21 (B) cells for 1 h at 4°C prior to virus addition. Approximately 1,000 FFU of ANDV were adsorbed for 1 h at 37°C to duplicate wells of a 96-well plate. After viral adsorption, inocula were removed, and the cells were washed and incubated 24 h at 37°C before methanol fixation. ANDV-infected cells were identified by immunoperoxidase staining of the viral nucleocapsid protein and quantitated by microscopy as previously described (38). The results were reproduced in at least two separate experiments and are presented as a percentage of the controls.