FIG. 5.

Human β₃ integrin PSI domain polypeptides inhibit ANDV infection. Increasing amounts (5 to 20 μg) of expressed, and purified polypeptides containing residues 1 to 53 of human or murine β₃ integrins were incubated for 2 h at 4°C with ∼1,000 FFU of ANDV. Virus was subsequently adsorbed to HUVECs (A) or BHK-21 cells (B) in 96-well plates for 1 h at 37°C. Monolayers were washed with PBS, and at 1 day postinfection the number of ANDV-infected cells was quantitated after methanol fixation and immunoperoxidase staining of the hantavirus nucleocapsid protein as previously described (38). The results were reproduced in at least two separate experiments and are presented as a percentage of the mock-treated controls.