FIG. 9.

P33L Mutants of the bovine β₃ integrin inhibit ANDV infection. Increasing amounts (5 to 20 μg) of human, murine, bovine (Bo) wild-type, or bovine P33L β₃ integrin PSI domain were incubated with ∼1,000 FFU of ANDV for 2 h at 4°C. Mixtures were adsorbed onto BHK-21 or VeroE6 cells in a 96-well plate for 1 h at 37°C. After a washing step, the cells were incubated for 24 h, and the number of ANDV-infected cells was quantitated by immunoperoxidase staining of the nucleocapsid protein as previously described (38). The results were reproduced in at least two separate experiments and are presented as a percentage of the mock-treated controls.