FIG. 3.

MDA5-deficient mice have increased susceptibility to lethal infection with CVB3. (A) MDA5^−/− mice (n = 6) and age- and sex-matched wild-type controls on a pure 129 SvJ background (n = 7) were injected i.p. with 10⁴ PFU of CVB3 and then monitored for survival. A total of 50% of MDA5^−/− mice died, whereas all of the wild-type mice survived more than 14 days (P = 0.03). (B) Histopathology of a liver from a MDA5^−/− mouse at 4 days postinfection reveals severe hepatic necrosis. Necrotic hepatocytes stain bright red. Brown granules overlying necrotic cells represent acid hematin. Magnification, ×400.