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ICP27 binds UL47 directly. Lysates were prepared from HeLa cells transfected with various expression vectors, indicated on top, and subjected to anti-Flag (A) or anti-ICP27 IP (B) (antibodies used for IP are shown in the shaded box). Coprecipitated proteins were resolved by 4 to 12% PAGE and probed with the indicated antibody. Protein content in lysates prior to IP is shown in input lanes. mIgG, mouse IgG; ab, antibody.
