Skip to main content
. 2009 Oct 21;84(1):426–436. doi: 10.1128/JVI.00725-09

FIG. 2.

FIG. 2.

MKRN1 reduces WNV-induced cell death. (A) Reduction of WNV-induced cell death in MKRN1 stable cell line. To assess the effects of MKRN1 overexpression against WNV cytotoxicity, U2OS cells constitutively expressing MKRN1 or a control were seeded at 8 × 105 in a 60-mm-diameter plate and infected with WNV at a multiplicity of infection of 1 PFU/cell. After 72 h, the cells were fixed, followed by PI staining. The stained cells were detected and analyzed via flow cytometry as described for Fig. 1C. (B) Ablation of MKRN1. U2OS cells were transfected using MKRN1 siRNA. After 72 h, the levels of the endogenous MKRN1 were measured using anti-MKRN1 antibodies. (C) Effects of MKRN1 depletion on WNV cytotoxicity. U2OS cells transfected with MKRN1 or control siRNA were seeded at 8 × 105 in a 60-mm-diameter plate and infected with WNV at a multiplicity of infection of 1 PFU/cell. After 72 h, the cells were fixed, followed by PI staining. The stained cells were detected and analyzed by flow cytometry.