FIG. 2.

Temporal expression of Hz-miR24 after HvAV-3e infection. (A) Predicted stem-loop secondary structure of pre-Hz-miR24 and mature Hz-miR24 sequences. (B) RT-qPCR analysis of Hz-miR24 expression at different time intervals following virus infection in HzFB cells. qPCR forward primers specific to Hz-miR24 and U6 were used, and the normalized expression of Hz-miR24 was calculated relative to that of U6. Error bars represent standard deviations from three replicates. (C) Northern blot analysis showing pre-miRNA and mature miRNA bands (arrows) using a reverse complementary probe to mature Hz-miR24 (upper) or Hz-miR29 (lower) as a control. tRNA is shown as a loading control. M, molecular size marker.