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(A) Cloning strategy to produce GFP-based reporter constructs with target and nontarget sequences. DdRP (RP), DdRPβ (β), and RNase III (RN; nontarget) were cloned downstream of the GFP open reading frame. (B) All three constructs were cotransfected individually with (+M24) or without (−M24) pIZ/pre-miR24 in HzFB cells, and the numbers of GFP-expressing cells were determined 72 h after the transfections. Error bars represent standard deviations from three independent determinations.
