FIG. 7.

Activation of SAPK decreases the transcriptional activity of Foxo3a. Wild-type C2C12 cells were cotransfected with either pGL3-Atrogin-1-Luciferase (Atrogin-1-Luc) or FHRE-luciferase (FHRE-Luc) and pCMV-β-galactosidase (βgal) plasmids. Two days later, C2C12 cells were treated with anisomycin (50 ng/ml) for the indicated times in the presence of LY294002 (20 μM). Emitted luminescence was measured on cell extracts, and Atrogin-1 or FHRE promoter activity corresponds to the ratio of Atrogin-1-Luc or FHRE-Luc activity to βgal activity (Luc/βgal). Results are represented as means ± standard deviations. *, significantly different from the control (LY294002; P < 0.001 [Student's t test]).