TABLE 2.
Plasmid constructs used in this study
| Plasmid | Main features | Source or reference |
|---|---|---|
| pJH33 | pRS316-HTA1-HTB1 HHT2-HHF2 | 1 |
| pJL51 | 2μm 3×HA-SGO1 | This study |
| pJL53 | ARS1 CEN4 URA3/pADH1-3×HA-SGO1-tADH1 | This study |
| pJL55 | pGEX-4T-1 3×HA-SGO1 | This study |
| pJL76 | ARS1 CEN4 URA3/pADH1-3×HA-SGO1-BD-tADH1 | This study |
| pMK439G44S | pRS315-HTA1-HTB1 hht2 G44S-HHF2 | This study |
| pMK572 | 2μm URA3 vector with ADH1 promoter and terminator | This study |
| pMK573 | 2μm URA3 SGO1 with ADH1 promoter and terminator | This study |
| pMK621 | pJJ244 URA3-HHF2-KTR5 insert | This study |
| pMK622G44S | pJJ244 hht2 G44S-HHF2-URA3-HHF2-KTR5 | This study |
| pMK622WT | pJJ244 HHT2-HHF2-URA3-HHF2-KTR5 | This study |
| pQQ18 | pRS315-HTA1-HTB1 HHT2-HHF2 | 1 |
| pYCF1/CEN3.L | YRp14/TEL cassette (pYCF1) with a CEN3 insert | 51 |