FIG. 5.

Ty1 mRNA/Gag foci are distinct from P-bodies. (A) Ty1 mRNA/Gag foci disassemble when P-bodies are activated. Wild-type (wt) cells were grown at 20°C in SC medium in the presence of glucose (+Glu) and then deprived of glucose (−Glu) for 25 min, which activates the formation of P-bodies. Ty1 mRNA and Gag were detected as described in the legend to Fig. 3B. (B) Levels of Ty1 mRNA in cells deprived of glucose. Northern blot analysis of Ty1 mRNA for the wild-type strain (BY4742) and isogenic HA-tagged derivatives grown in the presence of glucose (+Glu) and then deprived of glucose (−Glu) for 25 min. Equivalent amounts of total RNA were hybridized with a ³²P-labeled riboprobe corresponding to the 5′ end of GAG (see Fig. 2A). The signal detected for Ty1 mRNA was normalized to rRNA levels. Below the blot is the level of Ty1 mRNA without Glu relative to that of Ty1 mRNA with Glu.