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. 2009 Nov 9;30(2):382–398. doi: 10.1128/MCB.00251-09

FIG. 7.

FIG. 7.

P-body components are required for the formation of retrotransposition-competent VLPs. (A) P-body components are required for colocalization of Ty1 mRNA/Gag in foci after galactose induction of a pGTy1 plasmid. Cells overexpressing pGTy1 (DG2027, MAC350, MAC587, and MAC590) were induced for 16 h. Ty1 mRNA and Gag were detected as described in the legend to Fig. 3B. Merged mRNA/Gag and Gag/DAPI staining, DNA staining with DAPI, and corresponding DIC images are shown. The percentages of cells containing cytoplasmic Ty1 mRNA and Gag foci and the propensity of Ty1 mRNA/Gag foci or aggregates to colocalize and Gag/DAPI overlap are indicated. (B) Localization of VLPs by TEM in P-body component mutants overexpressing a pGTy1 plasmid after 16 h of galactose induction. Representative cells containing VLP clusters outlined in blue (right and middle panels) and dispersed VLPs indicated by yellow arrows (right panel) are shown. The black bars represent 500 nm.