FIG. 8.

P-body components are required for accumulation of IN and RT. Levels of IN, RT, and Gag in P-body component mutants. Total protein extracts from the wild type (wt) and P-body component mutants overexpressing pGTy1 for 16 h, extracted from equivalent cell densities, were subjected to Western blot analysis using anti-IN, anti-RT, and anti-Ty1 VLP antibodies. Comparable levels of protein were loaded, as judged from Coomassie blue staining. Protein molecular size standards (kilodaltons) are indicated.