FIG. 2.

Damage-induced phosphorylation of Rad26. (A) Western blot analysis of Rad26 from wild-type cells harvested either before (0) or at various time points after UV irradiation. (B) Western blot analysis of Rad26 immunoprecipitated before and 2 h after UV treatment and treated either with buffer alone or with shrimp alkaline phosphatase. (C) Western blot analysis of Rad26 from undamaged cells and from cells treated with the indicated doses of various DNA-damaging agents for 1 h. (D) Western blot analysis of Rad26 from untreated wild-type cells or from cells treated with heat shock (shift from 30°C to 42°C), osmotic shock (addition of 600 mM NaCl to the medium), or 6AU (addition of 200 μg/ml to the medium) for 1 h. (E) Western blot analysis of Rad26 after UV treatment in the presence of the translation inhibitor cycloheximide.