FIG. 2.
Loss of dyskerin and fibrillarin in hepatocyte nucleoli in Dkc1loxTg:Mx1Cre transgenic mice by immunohistochemistry. (A) Dyskerin localizes to the nucleolus and colocalizes with the C/D snoRNP protein fibrillarin. The liver tissue was paraffin embedded and incubated with rabbit antidyskerin and mouse antifibrillarin antibody. The nucleoli were counterstained with DAPI. (B) Western blotting showed that the amount of nuclear fibrillarin protein in the KO mice was not changed. TATA box binding protein (TBP) was used as a loading control.