Sequence alignment of TERT protein T motifs and hTERT T-motif mutants. (A) Alignment of TERT protein T-motif sequences from human (37, 40), dog (41), mouse (16), rat (GenBank accession no. AAF62177.1), hamster (18), chicken (11), frog (28), Moneuplotes crassus (26), Euplotes aediculatus (32), Oxytricha trifallax (6), Tetrahymena thermophila (6, 9), Schizosaccharomyces pombe (40), Encephalitozoon cuniculi (27), and Saccharomyces cerevisiae (32). The structural domain organization of TERT protein is shown above the sequence alignment. (B) Sequences of hTERT T-motif mutants studied in this work.