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. 2009 Nov 16;30(2):447–459. doi: 10.1128/MCB.00853-09

FIG. 4.

FIG. 4.

hTR binding by hTERT T-motif mutants. In vitro reconstitution of telomerase activity in the presence of radiolabeled hTR were carried out, followed by anti-FLAG immunoprecipitation (IP) of the TERT/TR complexes. The immunopurified products were analyzed via SDS-PAGE followed by phosphorimaging. The positions of hTR and hTERT are indicated. (A) Reconstitutions with radiolabeled hTR at a final concentration of hTR of 400 nM. (B) Reconstitutions with radiolabeled hTR at a final concentration of hTR of 40 nM.