TABLE 1.
Telomerase activities, repeat extension rates, and processivities of hTERT T-motif mutantsa
| hTERT | Relativeb telomerase activity (avg ± SD) | Extension ratec |
Processivityc |
||
|---|---|---|---|---|---|
| No. of repeats/min (avg ± SD) | Relativeb | Value (%) (avg ± SD) | Relativeb | ||
| WT | 1.00 | 6.79 ± 0.52 | 1.00 | 97.05 ± 0.79 | 1.00 |
| F560A | 0.41 ± 0.09 | 7.42 ± 0.12 | 1.09 | 98.26 ± 1.30 | 1.01 |
| F560Y | 0.40 ± 0.06 | 7.42 ± 0.14 | 1.09 | 98.29 ± 0.87 | 1.01 |
| F561A | 0.09 ± 0.01 | 6.83 ± 0.71 | 1.01 | 98.03 ± 1.19 | 1.01 |
| Y562A | 0.49 ± 0.01 | 12.78 ± 1.27 | 1.88 | 98.06 ± 1.61 | 1.01 |
| Y562F | 1.09 ± 0.18 | 12.50 ± 1.41 | 1.84 | 97.97 ± 0.55 | 1.01 |
| Y562T | 0.76 ± 0.05 | 11.08 ± 1.61 | 1.63 | 98.14 ± 0.32 | 1.01 |
| V563A | 0.31 ± 0.05 | 6.42 ± 0.59 | 0.94 | 98.40 ± 0.44 | 1.01 |
| V563L | 0.27 ± 0.04 | 9.92 ± 0.35 | 1.46 | 98.28 ± 0.97 | 1.01 |
| V563S | 0.33 ± 0.07 | 6.58 ± 0.35 | 0.97 | 98.34 ± 1.22 | 1.01 |
| T564A | 1.38 ± 0.18 | 28.83 ± 1.92 | 4.25 | 97.47 ± 0.74 | 1.00 |
| T564S | 0.50 ± 0.03 | 8.42 ± 0.35 | 1.24 | 97.79 ± 0.59 | 1.01 |
| T564V | 0.79 ± 0.20 | 22.44 ± 0.86 | 3.30 | 97.57 ± 0.42 | 1.01 |
| E565A | 0.34 ± 0.05 | 15.92 ± 0.82 | 2.34 | 98.84 ± 1.09 | 1.02 |
| Del-T | 0.00 | ND | ND | ||
| Sub-T | 0.00 | ND | ND | ||
a
Telomerase activities, extension rates, and processivities were determined from competitive primer challenge assays. Telomerase activities and extension rates represent averages of three independent experiments, while processivity values represent averages of two independent experiments.
b
Relative to that of the wild-type hTERT.
c
ND, not determined.