TABLE 2.
hTR binding by hTERT T-motif mutants
| hTERT | Relative bindinga (avg ± SD) with: |
|
|---|---|---|
| 400 nM hTR | 40 nM hTR | |
| WT | 1.00 | 1.00 |
| F560A | 1.09 ± 0.25 | 0.72 ± 0.06 |
| F560Y | 1.21 ± 0.09 | 0.94 ± 0.11 |
| F561A | 0.83 ± 0.06 | 0.35 ± 0.07 |
| Y562A | 1.06 ± 0.05 | 1.26 ± 0.09 |
| Y562F | 1.06 ± 0.20 | 1.39 ± 0.03 |
| Y562T | 0.93 ± 0.25 | 1.51 ± 0.04 |
| V563A | 1.16 ± 0.13 | 0.89 ± 0.08 |
| V563L | 1.25 ± 0.25 | 0.71 ± 0.17 |
| V563S | 1.35 ± 0.10 | 0.63 ± 0.02 |
| T564A | 1.11 ± 0.18 | 1.56 ± 0.06 |
| T564S | 1.08 ± 0.20 | 1.72 ± 0.25 |
| T564V | 0.94 ± 0.16 | 1.93 ± 0.56 |
| E565A | 1.03 ± 0.22 | 0.69 ± 0.07 |
| Del-T | 0.83 ± 0.08 | 0.36 ± 0.09 |
| Sub-T | 0.74 ± 0.13 | 0.35 ± 0.11 |
a
Relative to the wild type. Values represent averages of two independent experiments.