TABLE 3.
Extension rate of hTERT T-motif mutants reconstituted in the presence of MH3 template mutant hTR
| hTERT | Extension rate |
MH3hTRER/WThTRERb | |
|---|---|---|---|
| No. of repeats/min (avg ± SD) | Relativea | ||
| WT | 12.23 ± 1.28 | 1.00 | 1.80 |
| F561A | 12.99 ± 0.76 | 1.06 | 1.90 |
| Y562A | 23.43 ± 1.94 | 1.92 | 1.83 |
| Y562F | 21.90 ± 0.74 | 1.79 | 1.75 |
| Y562T | 19.05 ± 1.23 | 1.56 | 1.72 |
| T564A | 55.42 ± 6.12 | 4.53 | 1.92 |
| T564S | 14.77 ± 0.62 | 1.21 | 1.76 |
| T564V | 42.79 ± 1.17 | 3.50 | 1.91 |
| E565A | 30.36 ± 3.67 | 2.48 | 1.91 |
a
Relative extension rates are expressed relative to the rate for the wild-type hTERT.
b
Ratio of the extension rate with MH3 hTR to the extension rate with WT hTR (see Table 1). Rates represent averages of two independent experiments.