TABLE 4.
Extension rates of wild-type and T564A hTERT at different TTP concentrations
| TTP concn (μM) | Extension rate of the WT hTERT |
Extension rate of the T564A hTERT |
564AER/564TERb | ||
|---|---|---|---|---|---|
| No. of repeats/min (avg ± SD) | Relativea | No. of repeats/min (avg ± SD) | Relative | ||
| 5 | 0.82 ± 0.18 | 0.13 | 1.00 ± 0.15 | 0.04 | 1.22 |
| 10 | 1.27 ± 0.03 | 0.20 | 1.87 ± 0.22 | 0.07 | 1.47 |
| 20 | 1.73 ± 0.42 | 0.27 | 3.70 ± 0.32 | 0.13 | 2.13 |
| 50 | 2.90 ± 0.46 | 0.46 | 9.03 ± 0.41 | 0.32 | 3.11 |
| 100 | 3.47 ± 0.27 | 0.56 | 12.67 ± 1.90 | 0.45 | 3.65 |
| 1,000 | 6.23 ± 0.59 | 1.00 | 28.23 ± 1.61 | 1.00 | 4.53 |
a
Extension rates are expressed relative to the extension rate for 1,000 μM TTP for the given hTERT.
b
Ratio of the extension rate of 564A hTERT to the extension rate of 564T hTERT. The values are averages of two independent experiments.