. 2009 Nov 9;54(1):466–470. doi: 10.1128/AAC.00017-09

TABLE 2.

Kinetic parameters of β-lactamases VIM-1, VIM-2, and VIM-19^a

β-Lactam	Kinetic parameter of β-lactamase
	VIM-19			VIM-1^b			VIM-2^b
	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹·s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹·s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹·s⁻¹)
Benzylpenicillin	206	57	3.6	29	841	0.034	55.8	49	1.14
Cloxacillin	79.2	14	5.7	ND	ND		350	250	1.40
Amoxicillin	112	34	3.3	ND	ND		29.7	54	0.55
Ticarcillin	45.8	68	0.7	ND	ND		31.7	46	0.69
Piperacillin	160	37	4.3	1,860	3,500	0.53	32.7	72	0.45
Cephalothin	21.2	15	1.4	281	53	5.3	56.2	44	1.28
Cefoxitin	4.0	53	0.08	26	131	0.2	2.8	24	0.12
Cefuroxime	5.3	13	0.4	324	42	7.7	12.1	22	0.55
Aztreonam	<0.1			<0.01	>1,000		<0.5
Ceftazidime	6.3	88	0.07	60	794	0.076	88.7	98	0.90
Cefotaxime	34.3	36	0.95	169	247	0.68	27.5	32	0.86
Cefepime	12.5	55	0.23	549	145	3.8	4.7	184	0.03
Imipenem	8.2	22	0.37	2	1.5	1.3	9.9	10	0.99
Meropenem	1.3	9	0.14	13	48	0.27	1.4	5	0.28
Ertapenem	0.6	21	0.03	ND	ND		0.2	9	0.022

The standard deviation for analysis was ≤10%.

The kinetic parameters for VIM-1 are from Franceschini et al. (10) and those for VIM-2 are from Poirel et al. (21), except for cloxacillin and ertapenem, which are from Samuelsen et al. (28). ND, not determined.